Shimadzu HPLC systems demonstrate high reliability, with outstanding performance, such as ultra-low carryover and exceptional area reproducibility, and superior data quality. In addition, they are carefully designed for broad applicability and easy operation.
Higher throughput is of the utmost importance to laboratory efficiency and profitability. Nexera MX processes twice the number of samples as conventional LCMS systems in the same amount of time.
Improvement in the sensitivity of an LC/MS/MS method can generally be achieved by reducing the analytical scale of the analysis. However, reducing the analytical scale significantly to nano-flow reduces the throughput, stability, and robustness as a trade-off for greater sensitivity. Nexera Mikros, a Shimadzu microflow LC/MS system, offers a balanced solution to increased sensitivity with less compromise to throughput and robustness, using flow rates from 1 to 500 μL/min and column I.D. from 0.1 to 1 mm. It achieves stable and sensitive quantitative analysis through robust performance of microflow solvent delivery, optimized ion source design, the unique UF-Link column connection, and system variety that supports a wide range of applications.
The Nexera reducing sugar analysis system uses Shimadzu’s unique post-column boric acid-arginine fluorescence derivatization method. Unlike the refractive index detectors and evaporative light scattering detectors (ELSD) commonly used for sugar analysis, this system reduces the effects of impurities and offers both high sensitivity and a wide dynamic range. As a result, sugars contained in samples can be analyzed simultaneously, without dilution even when there are significant concentration differences.
Amino acid analysis occupies an important role in diverse fields such as food science and pharmaceutical manufacturing. Nexera Amino Acid Analysis System uses detection by post-column fluorescence derivatization, with o-phthalaldehyde (OPA)/N-acetylcysteine as the reaction reagents, to selectively and with high sensitivity quantitate the amino acids contained in samples with high levels of contaminants.
The Nexera Organic Acid Analysis System has been well-received in the fields of food and environment as a highly selective high-sensitivity system. It uses separation by ion exclusion chromatography and Shimadzu’s unique detection technique (pH-buffered electric conductivity detection). Recently, this system is being utilized by even more users in a wide variety of fields, and its usage in the fields of pharmaceuticals and life science is increasing.
Method Scouting System is a method development system based on Shimadzu’s UHPLC technology. The combination with Method Scouting Solution dedicated control software achieves a fast and accurate method scouting workflow, offering excellent support for method development.
Two major problems exist with traditional amino acid analysis techniques. The first technique (ion-exchange chromatography with postcolumn derivatization) allows many components to be analyzed at once but the method run times are long. The second technique (reverse-phase HPLC with pre-column derivatization) enables faster analysis but the number of analyzed components is limited. As a result, chemists are forced to make a decision to either analyze a large number of components or have a fast method.
The comprehensive 2D-LC methodology is a paradigm shift in liquid chromatography separation analysis. By combining two independent separation modes orthogonally in combination with a dual-loop/dual-valve alternate switching design, the highest possible peak capacity is achieved for LC chromatography separation. The new Nexera-e enables separation of even the most complex mixtures, providing a new level of knowledge and understanding of sample analytes. This is especially beneficial to the analysis of pharmaceutical impurities, proteolytic digests, food extracts, natural products and synthetic polymers.
Taking Online Dissolution Testing Into the New Era of Ultra High Performance Chromatography Nexera FVDissolution tests are widely used in original pharmaceuticals development and quality control and for bioequivalence tests in generic drugs.
The Co-Sense series features automatic sample processing systems intended to improve the efficiency of LC-MS operations by automating complicated sample processing steps. This eliminates the need for tedious, time-consuming, and expensive manual processes. It also achieves parameter settings for separating target components by HPLC can be set freely without being encumbered by LC-MS limitations. The Co-Sense series is especially well suited to the structural analysis of impurities in pharmaceuticals. It is also advantageous for LCMS measurement in the life sciences or chemical industry fields, such as to research metabolites in biological organisms or to analyze the side reaction in chemical synthesis.
The FDA draft guidance, "Genotoxic and Carcinogenic Impurities in Drug Substances and Products: Recommended Approaches" prescribes permitted limits for genotoxic impurities in drug substances. These impurities demand more sensitive trace-level analysis than normal impurities. Generally, mass spectrometry methods, such as GC/MS or LC/MS, are used for the high-sensitivity analysis of impurities. However, there is increasing demand for the establishment of a high-sensitivity quantitation method using an absorbance or other conventional detector that is easy to operate and can easily apply existing LC analysis conditions.
In LC-MS, there are various factors which may preclude the applicability of HPLC with MS analysis, including the interference associated with non-volatile buffers often used in HPLC. Therefore, tedious procedures are adopted, in which separation and collection is conducted by HPLC, and after desalting and other processing, MS measurement is performed.
Screening of 24 Synthetic Antimicrobial Compounds That Remain in Meat Synthetic antimicrobial agents are used as animal drugs and feed additives and they are known to remain in the body of farm animals. Residue limits prescribing the safe ingestible amounts of such agents have been set so that people can safely eat meat and processed meat products. Thanks to a pretreatment method*1 that minimizes the influence of impurities in meat (beef, pork, and chicken muscle), LC system enables screening of 24 synthetic antimicrobial compounds, which are regulated in Japan, Europe, and other regions, remaining in meat. *1: Consumables for pretreatment are not included in the package. They need to be provided by the customer.
Mycotoxin (mold toxin) is a generic term for metabolites produced by molds on food products that are hazardous to human and animal health. To improve food safety, food processors have been inspecting ingredients for the presence of such mycotoxins. In only 14 minutes, this mycotoxin screening system is able to detect the presence of 10 mycotoxin components with high sensitivity at concentration levels specifled by EU standards, which are the strictest in the world. Furthermore, because the system does not involve a sample derivatization process, samples can be measured much more efficiently.
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