ThermaGenix Inc.
ThermaGenix is committed to empowering scientists all over the world with the highest performing PCR additives. Our innovative PCR additives improve polymerase specificity and fidelity, enhancing end point data and achieving superior amplicons for downstream applications. From genotyping to Gibson assembly, next generation sequencing sample preparation to diagnostics, ThermaGenix technology will maximize PCR performance.
- 507-639-8727
- 16 Tech Circle
Suite 201
Natick, MA 01760
United States of America
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Product
Reverse Transcription and Polymerase
ThermaStop-RT
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RNA oligonucleotides from cells and viruses can be amplified for detection using the methods of Reverse Transcription and Polymerase Chain Reaction (RT-PCR). In a first step, a complementary strand of DNA (cDNA) is synthesized using a primer and reverse transcriptase. The cDNA is then amplified using standard PCR techniques. This can be accomplished by taking an aliquot from the reverse transcription reaction and adding a DNA-dependent DNA polymerase (e.g. Taq) and other reagents and then doing PCR. That method is commonly called "Two-step RT-PCR", or "Two-tube RT-PCR". Alternatively, all reagents, including both enzymes and gene-specific primers can be included in a single tube in order to do both reverse trancription and PCR without the need to open the tube between those reactions. That method is commonly called "One-Step RT-PCR" or "One-Tube RT-PCR". Both methods can include primers for a single target or for multiple targets (i.e. multiplex RT-PCR).
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Product
A Universal Reagent
ThermaStop™
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ThermaStop maximizes PCR performance, improves polymerase specificity, enhances end quantitative data and achieves superior amplicon yield for downstream applications from genotyping to Gibson assembly, next generation sequencing sample preparation to diagnostics.
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Product
Enhanced Taq DNA Polymerase
ThermaGo™
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ThermaGo™ is a double-stranded, chemically modified nucleic acid that suppresses mis-priming during the annealing and extension steps of PCR. It increases the reproducibility of both real-time and end-point analyses. ThermaGo enhances the specificity of Taq DNA polymerase.
