Becker & Hickl GmbH
Becker & Hickl have introduced a proprietary time-correlated single-photon counting principle that made TCSPC more than 100 times faster than the existing devices. Moreover, bh introduced a multi-dimensional TCSPC process that records the photons not only versus the time in the signal period, but also versus other parameters, such as experiment time, wavelength, or spatial coordinates. The bh devices are designed to record multi-dimensional photon distributions, time-resolved images, sequences of photon distributions, or multi-dimensional time-tag data. The bh TCSPC products are complemented by bh picosecond diode lasers, detector modules, multi-spectral detector assemblies, and experiment control modules. Based on these components Becker & Hickl supply their own confocal fluorescence lifetime laser scanning microscope and FLIM upgrade kits for laser scanning microscopes of various manufacturers. Moreover, bh is supplier of TCSPC modules for commercial time-domain optical tomography instruments. bh market activities include currently 5 workshops around the world and distribution of more than 1200 pages of TCSPC literature.
- +49 (30) 212 80 02-0
- +49 (30) 212 80 02-13
- info@becker-hickl.com
- Nunsdorfer Ring 7-9
Berlin, 12277
Germany
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product
Laser
BDS-SMY Series
*Pulse Width Down to 50 ps*Repetition Rate 50 MHz (20 MHz Available on Request)*Free Beam or Fiber Output*CW-Equivalent Power 0.3 mW to 0.5 mW*All Electronics Integrated*No External Driver Unit*Simple +12 V Supply*40 mm x 70 mm x 120 mm
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FLIM Upgrades for Leica
SP2 / SP5 / SP8
*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Identical Systems for Multiphoton FLIM and Confocal FLIM*Detectors can be Swapped Between RLD Port and X1 Port*Systems are Modular: More FLIM Channels can be Added
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FLIM Upgrade Kits for LSMs
As technology leader in equipment and techniques for single photon counting, Becker & Hickl offers a wide range of high-grade Fluorescence Lifetime Imaging (FLIM) systems for laser scanning microscopes since 1998. Our techniques have found broad application within the scope of Life Sciences, Clinical FLIM, Diffuse Optical Tomography (DOT), Fluorescence Correlation (FCS, FCCS), Förster Resonance Energy Transfer (FRET) and more.
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product
Multiphoton FLIM System
DCS-120 MP
*Multiphoton FLIM Upgrade for Existing Conventional Microscope*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Scanning by Fast Galvanometer Mirrors*Channel Seperation by Dichroic or Polarising Beamsplitters*Individually Selectable Pinholes and Filters*Excitation by Ti:Sa Laser*Two Non-Descanned Detection Channels*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: Two HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Optional: HPM-100-50 Hybrid Detectors for NI...show more -
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Analysis Software for FLIM, PLIM
SPCImage
*Analysis of Single-Decay, FLIM and PLIM Data*Analysis by Iterative Convolution and Fit Procedure or by First Moment of Photon Distribution*Calculations of Decay Parameters and Lifetimes by Various Decay Models*Calculation of FRET Efficiencies*Display of Lifetime and FRET Images*Display of Lifetimes, Amplitudes, Intensities or Ratios*Export of Lifetime Data and Images
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Laser Scanning FLIM Microscopes
DCS-120 laser scanner: Compact - Flexible - Precise. As technology leader in equipment and techniques for single photon counting, Becker & Hickl offers the DCS-120 systems that are complete laser scanning microscopes for fluorescence lifetime imaging (FLIM) since 2007. The systems use bh’s multi-dimensional TCSPC FLIM technology in combination with fast laser scanning and confocal detection or multi-photon excitation.
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product
Confocal FLIM System
DCS-120
*FLIM Upgrade for Existing Conventional Microscope*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Scanning by Fast Galvanometer Mirrors*Channel Seperation by Dichroic or Polarising Beamsplitters*Individually Selectable Pinholes and Filters*One or Two BDL-SMN or BDS-SM ps Diode Lasers*Two Fully Confocal Detection Channels*Two HPM-100-40 GaAsP Hybrid Detectors*Optional: Two HPM-100-06 Hybrid Detectors, IRF Width < 20 ps FWHM*Optional: HPM-100-50 Hybrid Detector...show more -
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Fiber-Based TCSPC System
*Hybrid Fiber System With Implantable Fiber Tip*Tip Removable from Fiber System*Seperate Excitation and Detection Fibers*Extremly Low Background Fluorescence*One or Two bh ps Diode Laser (BDS-SM or BDL-SMN Series)*High-Efficiency HPM-100-40 or -50 Hybrid Detector*Optional: Multi-Wavelength Detector
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FLIM Upgrades for Nikon
A1 / C1 / C2
*bh FLIM Embedded in Nikon NIS-Elements Software*Two Fully Parallel TCSPC FLIM Channels*Compact bh Simple-Tau or Power-Tau System*Two HPM-100-40 GaAsP Hybrid Detectors*Excitation by bh ps Diode Lasers*Excellent Time Resolution: Electrical IRF Width 6.5 ps FWHM*Excellent Timing Stability*Time Channel Width Down to 813 fs*Megapixel FLIM, Up to 2048 x 2048 Pixels at 256 Time Channels*Detection Wavelength Range from 360 nm to 700 nm
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product
Laser
BDS-MM Series
*Pulse Width Down to 60 ps*Repetition Rate 20 MHz / 50 MHz*Free Beam or Fiber Output*Optical Power Up to 40 mW*All Electronics Integrated*No External Driver Unit*Simple +12 V Supply*40 mm x 70 mm x 120 mm
